2019-08-05 13:54:332025-04-07 14:11:03
description
class A penicillin-binding protein 1A/1B, contributes to cell elongation and [SW|cell division]
class A penicillin-binding protein 1A/1B, contributes to cell elongation and [SW|cell division], required for the control of cell diameter (together with the [SW|Rod complex])
function
bifunctional glucosyltransferase/ transpeptidase
bifunctional glucosyltransferase/ transpeptidase, polymerizes and cross-links peptidoglycan
Gene
Phenotypes of a mutant
prevents bulging of the cells when grown at low Mg(2 ) concentrations, suppresses the lethal effect of a [[gene|mreB]] mutation [Pubmed|19192185]
deletion of [[gene|ponA]] restores growth and normal shape of a [[gene|glmR]] mutant on gluconeogenic carbon sources [Pubmed|21320184]
a strain lacking all four class A [SW|penicillin-binding proteins] ([[gene|ponA]] [[gene|pbpD]] [[gene|pbpF]] [[gene|pbpG]]) is severely inhibited for L-form switching in the presence of D-cycloserine [pubmed|29456081]
prevents bulging of the cells when grown at low Mg2+ concentrations, suppresses the lethal effect of a [[gene|mreB]] mutation [Pubmed|19192185]
deletion of [[gene|ponA]] restores growth and normal shape of a [[gene|glmR]] mutant on gluconeogenic carbon sources [Pubmed|21320184]
a strain lacking all four class A [SW|penicillin-binding proteins] ([[gene|ponA]] [[gene|pbpD]] [[gene|pbpF]] [[gene|pbpG]]) is severely inhibited for L-form switching in the presence of D-cycloserine [pubmed|29456081]
The protein
Catalyzed reaction/ biological activity
[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+
[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)
contributes to the control of cell diameter (cell diameter increases with PonA levels), acts antogonistically to the [SW|Rod complex] [pubmed|31086310]
References
Original publications
The protein
[SW|Domains]
Fibronectin type-III (aa 708-795) (according to UniProt)